BIOCHEMICAL TECHNIQUES:: PART 2

CENTRIFUGATION

INTRODUCTION

-         This method of separation is based on sedimentation rate.

-         Sedimentation rate depends upon the size and shape of the molecule and the viscosity of the solution.

-         Higher the molecular weight greater the rate of sedimentation.

-         In centrifugation we rotate a suspension at a very high speed so that the components settle down quickly.

                              

-         When a suspension is centrifuged the components of the suspension of the suspension are separated into pallets and supernatant.

-         Every suspension has to be centrifuged at a specific speed for a specific time for its separation.