Types of
Centrifugation Based On Speed (RPM)
-
Low speed centrifugation
o
RPM
is between 3000-6000.
o
RCF
is 6000g.
o
Large
organelles like nucleus and chloroplast are separated.
-
Micro speed centrifugation
o
RPM
is 12000.
o
RCF
is 10000g.
-
High speed centrifugation
o
RPM
is 25000.
o
RCF
is 60000g.
o
Used
to separate mitochondria and lysosomes.
-
Ultracentrifugation
o
RPM
is 30000.
o
RCF
id 60000g.
o
Used
to separate ribosomes, vesicles and other biological macromolecules.
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