SEMI-CONSERVATIVE MODE OF DNA REPLICATION
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This is called semi conservative because the new
strand of DNA contains one parental strand and one newly synthesized strand.
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EXPERIMENT
o N15
is a
heavy isotope of nitrogen.
o E.coli was grown
in a nutrient medium that contained N15 instead of N14.
o Hence N15
got
incorporated into the purines and pyrimidines bases of the DNA.
o After this
E.coli were removed from N15 medium and kept in N14 medium.
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