BIOCHEMICAL TECHNIQUES:: PART 8

WORKING

-         This technique uses a medium called Agarose gel.

-         The DNA fragments are digested with the help of restriction enzymes and placed in the wells made in the gel setup.

-         An electric field is applied across the gel.

-         Since the DNA molecule is negatively charged it moves towards the positive electrode.

                             

-         The smallest strand of DNA covers the maximum distance in a given period of time than the larger strands.

-         The DNA strands can be stained with Ethidium bromide and visualized.